https://test.sciltp.com/testj/jmnp/issue/feed 2024-12-06T00:00:00+08:00 Mr. Zack Li jmnp@sciltp.com Open Journal Systems https://test.sciltp.com/testj/jmnp/article/view/353 2024-04-09T13:30:45+08:00 Lutfun Nahar L.Nahar@ljmu.ac.uk

<p class="categorytitle"><em>Editorial</em></p> <h1>Journal of Medicinal Natural Products: A New Journal with a New Perspective</h1> <div class="abstract_title"> <p><strong>Lutfun Nahar ^1,2</strong></p> </div> <div class="abstract_top"> <p>1 Centre for Natural Products Discovery, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, UK; L.Nahar@ljmu.ac.uk or nahar@ueb.cas.cz</p> <p>2 Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany, The Czech Academy of Sciences, Šlechtitelů 27, 78371 Olomouc, Czech Republic</p> <p>Received: 7 April 2024; Accepted: 20 April 2024; Published: 14 May 2024</p> </div>

2024-05-14T00:00:00+08:00 Copyright (c) 2024 by the authors. https://test.sciltp.com/testj/jmnp/article/view/352 2024-04-25T10:49:34+08:00 Chuanchom Khuniad C.Khuniad@2020.ljmu.ac.uk Lutfun Nahar L.Nahar@ljmu.ac.uk Jason W. Birkett J.W.Birkett@ljmu.ac.uk Kenneth J. Ritchie K.J.Ritchie@ljmu.ac.uk Satyajit D. Sarker S.Sarker@ljmu.ac.uk

<p class="categorytitle"><em>Article</em></p> <h1>Composition of <em>Amesiodendron chinense</em> (Merr.) Hu Seed Oil and Assessment of Its Nrf2/ARE Induction Activity in AREc32 Cells</h1> <div class="abstract_title"> <p><strong>Chuanchom Khuniad</strong><strong> </strong><strong>^1,2,*</strong>, Lutfun Nahar</strong><strong> </strong><strong>^3,*</strong><strong>, Jason W. Birkett </strong><strong>⁴</strong><strong>, Kenneth J. Ritchie</strong><strong> </strong><strong>¹</strong><strong> and</strong><strong> </strong><strong>Satyajit D. Sarker</strong><strong> </strong><strong>¹</strong><strong>^,*</strong></p> </div> <div class="abstract_top"> <p>¹ Centre for Natural Products Discovery (CNPD), School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK </p> <p>² Department of Thai Traditional Medicine, Faculty of Health and Sports Science, Thaksin University, Phatthalung 93210, Thailand</p> <p>³ Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany, The Czech Academy of Sciences, Šlechtitelů 27, 78371 Olomouc, Czech Republic</p> <p>⁴ Forensic Science Research Group, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK</p> <p><strong>*</strong> Correspondence: C.Khuniad@2020.ljmu.ac.uk (C.K.); nahar@ueb.cas.cz (L.N.) and S.Sarker@ljmu.ac.uk (S.D.S.)</p> <p>Received: 7 April 2024; Revised: 26 April 2024; Accepted: 6 May 2024; Published: 14 May 2024</p> <p> </p> </div> <p><strong id="abstract" class="label">Abstract:</strong> Background: Amesiodendron chinense (Merr.) Hu (family: Sapindaceae) is a Thai medicinal plant. The seed oil of this species has been used by folk healers and local people in southern Thailand for the treatment of wounds, skin disorders and common hair problems. This study aimed at the GC-MS-based determination of the chemical composition of the seed oil of this plant, and evaluation of its Nrf2/ARE induction activity in AREc32 cells (modified human breast cancer cell line MCF-7) using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and luciferase reporter gene assays. Results: GC-MS analysis identified 9-(E)-octadecenoic acid (84.82%) as the main component of this seed oil. TLC-based qualitative DPPH (2,2-diphenyl-1-picrylhydrazyl) assay revealed the DPPH radical-scavenging activity of the seed oil and its chromatographic fractions. A low-level DPPH-scavenging activity was observed in the quantitative assay, but no IC50 value could be determined even with the highest tested concentration (10 mg/mL). Neither the oil nor its chromatographic fractions showed any significant Nrf2/ARE induction in AREc32 cells. The seed oil was noncytotoxic against the AREc32 cells. Conclusions: A. chinense seed oil and its fractions had a low level of free-radical scavenging property but no significant Nrf2/ARE induction activity in AREc32 cells. However, as the oil did not show any cytotoxicity at test concentrations in the MTT assay, this oil might potentially be safe to use in cosmetic formulations or as a vehicle for the dermal delivery of drug molecules.</p>

2024-05-14T00:00:00+08:00 Copyright (c) 2024 by the authors. https://test.sciltp.com/testj/jmnp/article/view/357 2024-07-03T09:06:11+08:00 Arun Kumar Singh Parihar arunpharma1986@gmail.com Umakant Sahu uksahu28@gmail.com Kamleshwar Singh Karbhal dr.k.s.karbhal95@gmail.com Shrikant Rambhau Inchulkar SHRIKANTINCHULKAR24@gmail.com Nagendra Singh Chauhan chauhan.nagendra@gmail.com Mayank Krishna Kulshreshtha drmayankkrishnakulshrestha@gmail.com

<p class="categorytitle"><em>Article</em></p> <h1>Quality Control of Triphala Churna</h1> <div class="abstract_title"> <p><strong>Arun Kumar Singh Parihar</strong><strong> </strong><strong>¹</strong><strong>, Umakant Sahu</strong><strong> </strong><strong>¹</strong><strong>, Kamleshwar Singh Karbhal</strong><strong> </strong><strong>¹</strong><strong>, Shrikant Rambhau Inchulkar</strong><strong> </strong><strong>¹</strong><strong>, </strong><strong>Nagendra Singh</strong><strong> Chauhan</strong><strong> </strong><strong>^1,</strong><strong>*</strong><strong>, and </strong><strong>Mayank Krishna Kulshreshtha</strong><strong> </strong><strong>²</strong></p> </div> <div class="abstract_top"> <p>¹ Drugs Testing Laboratory AvamAnusandhan Kendra, Raipur, Chhattisgarh 492010, India</p> <p>² Government Ayurved College, Bilaspur, Chhattisgarh 495001, India </p> <p><strong>*</strong> Correspondence: chauhan.nagendra@gmail.com; Tel. +919406558176</p> </div> <div class="abstract_top"> <p>Received: 22 April 2024; Revised: 3 July 2024; Accepted: 15 July 2024; Published: 23 July 2024</p> <p> </p> </div> <p><strong id="abstract" class="label">Abstract: </strong>Objectives: Triphala Churna (TC) is a tridoshashamak Ayurvedic rasayana used for treating various diseases such as skin diseases, diabetes, and as a mild laxative. Methods: In this study, TC was prepared and quality standardized standardized using various parameters such as macroscopical evaluation, physical properties, physicochemical properties, and phytochemical analysis (HPTLC fingerprinting and quantification) methods to assess its quality and purity. Results: The qualitative and quantitative phytochemical assays via HPTLC revealed concentrations of Gallic acid as 3.62 ± 0.05%, 3.67 ± 0.04%, 1.52 ± 0.03%, and 2.96 ± 0.02% for Amlaki (<em>Emblica officinalis</em>), Haritiki (<em>Terminalia chebula</em>), Vibhitki (<em>Terminalia bellerica</em>), and TC, respectively. Conclusion: This study provides valuable insights into establishing robust quality control measures and developing reliable assays for both Triphala Churna (TC) and its individual components.</p>

2024-07-23T00:00:00+08:00 Copyright (c) 2024 by the authors. https://test.sciltp.com/testj/jmnp/article/view/493 2024-09-05T13:30:11+08:00 Mirza Alimullah mirza.alimullah@northsouth.edu Nafiur Rahman asm.nafiur@northsouth.edu Puspa Sornaker puspas402@gmail.com Kazi Akramuddaula akramuddaula@gmail.com Sumaia Sarif sumiajeeyon@gmail.com Shahnaz Siddiqua siddiqua.edu@gmail.com Kaniz Fatima Mitu kaniz.mitu@northsouth.edu Ishrat Jahan ishrat.jahan02@northsouth.edu Ferdous Khan khan.ferdous@northsouth.edu Nusrat Subhan nusrat.subhan@northsouth.edu Md. Ashraful Alam sonaliagun@yahoo.com

<p class="categorytitle"><em>Article</em></p> <h1>Evaluation of <em>Terminalia arjuna</em> Bark Powder Supplementation on Isoprenaline-Induced Oxidative Stress and Inflammation in the Heart of Long Evans Rats, Understanding the Molecular Mechanism of This Old Medicinal Plant</h1> <div class="abstract_title"> <p><strong>Mirza Alimullah ¹, Nafiur Rahman ¹, Puspa Sornaker ¹, Kazi Akramuddaula ², Sumaia Sarif ¹, Shahnaz Siddiqua ³, Kaniz Fatima Mitu ¹, Ishrat Jahan ¹, Ferdous Khan ¹, Nusrat Subhan ¹ and Ashraful Alam ¹^,*</strong></p> </div> <div class="abstract_top"> <p>¹ Department of Pharmaceutical Sciences, North South University, Dhaka 1229, Bangladesh</p> <p>² Pharmacy Discipline, Khulna University, Khulna 9208, Bangladesh</p> <p>³ Department of Pharmacy, East West University, Dhaka 1212, Bangladesh</p> <p><strong>*</strong> Correspondence: ashraful.alam@northsouth.edu or sonaliagun@yahoo.com</p> </div> <div class="abstract_top"> <p>Received: 27 July 2024; Revised: 28 August 2024; Accepted: 28 August 2024; Published: 5 September 2024</p> <p> </p> </div> <p><strong class="label">Abstract: </strong>This study was conducted to determine the effect of <em>Terminalia arjuna</em> bark powder supplementation on the oxidative stress of the cardiovascular system. Isoprenaline (ISO) was administered to the rats to develop the cardiac hypertrophy and myocardial infarction (MI). <em>Terminalia arjuna</em> bark powder was mixed with the food powder and provided for two weeks. At the end of the experiment, all rats were sacrificed and tissue samples were collected. ISO administration in rats increased the oxidative stress markers such as malondialdehyde (MDA), nitric oxide (NO), advanced oxidation protein product (APOP), and myeloperoxidase (MPO) in plasma and heart. <em>Terminalia arjuna</em> bark powder lowered the MDA, NO, and AOPP concentration level in ISO administered rats. Additionally, <em>Terminalia arjuna</em> restored the antioxidant enzymes (catalase and SOD) activities. Gene expression of antioxidant enzymes and inflammatory markers in the heart were studied. <em>Terminalia arjuna</em> restored Nrf-2, HO-1, HO-2, catalase, SOD, and GPx gene expression in the heart of ISO administered rats. ISO induced increased transcription levels of inflammatory genes such as IL-1, IL-6, TNF-α, TGF-β, iNOS, and NF-κB, which were decreased by <em>Terminalia arjuna</em> bark powder. Histopathology was checked and hematoxylin and eosin and Sirius red staining were performed on heart sections. ISO administration resulted in mononuclear cells infiltration and collagen deposition in the heart which were lowered by <em>Terminalia arjuna</em> bark powder. In conclusion, this study suggests that the <em>Terminalia arjuna</em> bark powder alleviated the oxidative stress by restoring the antioxidant genes and prevented the increase in inflammatory markers in the heart of ISO administered rats.</p>

2024-09-05T00:00:00+08:00 Copyright (c) 2024 by the authors. https://test.sciltp.com/testj/jmnp/article/view/405 2024-09-23T10:10:47+08:00 Holly Siddique h.siddique@herts.ac.uk Mukhleshur Rahman m.rahman@uel.ac.uk

<p class="categorytitle"><em>Article</em></p> <h1>Phytochemical Analysis of Bangladeshi Medicinal Plants Led to the Isolation of Anti-Staphylococcal Compounds</h1> <div class="abstract_title"> <p><strong>Holly Siddique ^1,2,* and Mukhleshur Rahman ³</strong></p> </div> <div class="abstract_top"> <p>¹ Department of Clinical and Pharmaceutical Science, School of Life and Medical Science, University of Hertfordshire, College Lane, Hatfield AL10 9AB, UK</p> <p>² Royal Botanic Gardens Kew, Kew Green, Richmond, Surrey TW9 3AE, UK</p> <p>³ Medicines Research Group, School of Health, Sports and Bioscience, University of East London, Stratford Campus, Water Lane, London E15 4LZ, UK</p> <p><strong>*</strong> Correspondence: h.siddique@herts.ac.uk</p> </div> <div class="abstract_top"> <p>Received: 20 June 2024; Revised: 26 August 2024; Accepted: 12 September 2024; Published: 24 September 2024</p> <p> </p> </div> <p><strong class="label">Abstract: </strong>Antibacterial resistance is a major threat to global health. Due to its new resistance mechanisms, it is spreading and emerging widely, thereby threatening the treatment of common infectious diseases. Ancient history and ethnopharmacological studies highlighted the importance of natural sources in treating resistance infections. This study involved bioassay-directed phytochemical investigation on Bangladeshi medicinal plants selected by an ethnopharmacological survey to explore antibacterial compounds against Methicillin resistance <em>Staphylococcus aureus</em> (MRSA). In 2016, an ethnopharmacological survey conducted in Bangladesh led to the recommendation of 71 medicinal plants by 127 respondents (71 Ayurvedic/Unani practitioners, 21 Ayurvedic patients and 35 local inhabitants) for the treatment of infectious diseases. Based on the literature review, data analysis of the ethnopharmacological survey and ease of availability of the plants, 18 plants were initially selected and collected from Bangladesh. After the initial antibacterial screening of 18 plants, five plants with Minimum Inhibitory Concentration (MIC) of 32–512 µg/mL were chosen based on potential antibacterial activity. These are (<em>Zingiber montanum</em>, <em>Uraria picta</em>, <em>Diospyros malabarica</em>, <em>Cynometra ramiflora</em>, <em>Swertia chirayita</em>. Extensive phytochemical work using different chromatographic and spectroscopic techniques on five Bangladeshi medicinal plants led to the isolation and identification of 24 compounds. Eight terpenes (zerumbol (<strong>3</strong>), zerumbone (<strong>4</strong>), buddledone A (<strong>5</strong>), germacrone (<strong>6</strong>), furanodienone (<strong>7</strong>), (−) borneol (<strong>1</strong>)<strong>,</strong> camphor (<strong>2</strong>) and 8(17), 12-labdadiene-15, 16-dial) (<strong>8</strong>)<strong> </strong>were isolated from <em>Zingiber montanum </em>with the MIC (32– &gt;128 µg/mL). Eugenol (<strong>14</strong>) and steroids were isolated from <em>Uraria picta </em>(MIC 64– &gt;128 µg/mL). Lupane-type triterpenoids (Lupeol (<strong>20</strong>), betulin (<strong>21</strong>)<strong>,</strong> betulinaldehyde (<strong>23</strong>)<strong>,</strong> betulone (<strong>24</strong>) and messagenin (<strong>22</strong>) were isolated and identified from <em>Diospyros malabarica </em>with the MIC (64– &gt;128 µg/mL), while pentacyclic triterpene (glutinol (<strong>10</strong>)<strong>,</strong> glutinone (<strong>11</strong>)), simple phenolic (ethyl 4-ethoxybenzoate (<strong>9</strong>)) and steroids were isolated from <em>Cynometra ramiflora </em>with MIC (64– &gt;128 µg/mL). A series of xanthones (swerchirin (<strong>16</strong>), swertiaperenin (<strong>17</strong>), bellidifolin (<strong>18</strong>) and decussatin (<strong>19</strong>)) were identified from <em>Swertia chirayita </em>with MIC (&gt;128 µg/mL). 4-ethoxybenzoate (9) and messagenin (22) were identified as new natural compounds among these compounds. In terms of activity, 8(17), 12-labdadiene-15, 16-dial (<strong>8</strong>) (32 µg/mL against ATCC 5941) and zerumbol (<strong>3</strong>) (32 µg/mL against EMRSA 15) exhibited potential antibacterial activity. Phytochemical discoveries of Bangladeshi medicinal plants gave a new dimension to exploring anti-staphylococcal compounds.</p>

2024-09-24T00:00:00+08:00 Copyright (c) 2024 by the authors. https://test.sciltp.com/testj/jmnp/article/view/556 2024-10-16T13:39:13+08:00 Muhammad Shehu Giwa kasumscbio180016@kasu.edu.ng Basira Ibrahim basira.ibrahim@kasu.edu.ng Fatima Musa fatima.mohammed@kasu.edu.ng Emad M. Abdallah emad100sdl@yahoo.com

<p class="categorytitle"><em>Article</em></p> <h1>Unveiling the Bioactive Phytochemicals of <em>Momordica charantia</em> Leaves and Their Antibacterial Effects</h1> <div class="abstract_title"> <p><strong>Muhammad Shehu Giwa ¹, Basira Ibrahim ¹, Fatima Musa ¹ and Emad M. Abdallah ^{2,3,* <a href="https://orcid.org/0000-0003-0549-249X" target="_blank" rel="noopener"><img style="position: relative; width: 20px; margin-left: 3px; max-width: 20px !important; height: auto; top: 5px;" title="ORCID" src="https://www.sciltp.com/journals/public/site/images/orcid.png" /></a>}</strong></p> </div> <div class="abstract_top"> <p>¹ Department of Biological Sciences, Faculty of Life Sciences, College of Science, Computing and Engineering, Kaduna State University, 800283 Kaduna, Nigeria</p> <p>² Department of Biology, College of Science, Qassim University, Qassim 51452, Saudi Arabia</p> <p>³ Faculty of Health and Life Sciences, INTI International University, Persiaran Perdana BBN, Putra Nilai, 71800 Nilai, Negeri Sembilan, Malaysia.</p> <p><strong>*</strong> Correspondence: emad100sdl@yahoo.com</p> </div> <div class="abstract_top"> <p>Received: 21 September 2024; Revised: 9 October 2024; Accepted: 11 October 2024; Published: 16 October 2024</p> <p> </p> </div> <p><strong class="label">Abstract: </strong><em>Momordica charantia </em>is widely cultivated in Zaria, Nigeria, and holds a prominent place in traditional medicine. Its leaves, fruits, and seeds are known to be rich in bioactive compounds and are commonly employed to treat various infections and diseases. This study aimed to investigate the bioactive components and antibacterial properties of methanolic leaf extracts and their fractions. Phytochemical analysis of the methanolic extract revealed the presence of alkaloids, saponins, flavonoids, glycosides, tannins, steroids, and terpenoids. The methanolic extract was fractionated into n-butanol, ethyl acetate, and chloroform fractions. The methanolic extract exhibited superior antibacterial activity compared to its fractions, suggesting potential synergistic effects among the plant’s constituents. Antibacterial efficacy was evaluated using well-diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. <em>Salmonella typhi</em> was the most susceptible bacterium, with zones of inhibition of 25.00 ± 0.10 mm, MIC of 12.5 ± 0.82 mg/mL, and MBC of 50 ± 0.22 mg/mL. This was followed by <em>Escherichia coli</em> (18.77 ± 0.25 mm, MIC: 50 ± 0.53 mg/mL, MBC: 100 ± 0.82 mg/mL) and <em>Staphylococcus aureus</em> (14.13 ± 0.91 mm, MIC: 50 ± 0.23 mg/mL, MBC: 100 ± 0.48 mg/mL). Among the fractions, the n-butanol fraction demonstrated the highest antibacterial activity. Subsequent analysis of this fraction using GC-MS identified key compounds, including 2-pentanone, 4-hydroxy-4-methyl-, n-amyl isovalerate, 2(5<em>H</em>)-furanone, 3,5,5-trimethyl-, furan, tetrahydro-2,2,4,4-tetramethyl-, and 3-tetradecanol acetate. In conclusion, the methanolic extract followed by n-butanol fraction of <em>M. charantia</em> exhibited significant antibacterial activity, particularly against Gram-negative bacteria such as <em>S. typhi</em> and <em>E. coli</em>. Further research is recommended to isolate and characterize the bioactive compounds responsible for this activity.</p>

2024-10-16T00:00:00+08:00 Copyright (c) 2024 by the authors. https://test.sciltp.com/testj/jmnp/article/view/623 2024-11-27T14:46:58+08:00 Md. Shahlal sahlalbiswas61@gmail.com As-Sazzad Mahmud sazzadnishan96@gmail.com Rahul Dev Bairagi rahuldev131133@gmail.com Dipa Debnath dipa.ku.ph@gmail.com Barsha Sarker Nipa borshasarker76@gmail.com Raiyan Rahman Reon drzreon@gmail.com Rony Ahmed ahmed.rony888@gmail.com Tawhidur Rahman tawhidur257@gmail.com Shankar Sharma shankarsharma8810@gmail.com Amit Kumar Acharzo amitproduth007@gmail.com

<p class="categorytitle"><em>Article</em></p> <h1>Evaluation of Therapeutic Activity of <em>Physalis angulata</em> (In Vitro Studies)</h1> <div class="abstract_title"> <p><strong>Md. Shahlal </strong><strong>¹</strong><strong>, As-Sazzad Mahmud </strong><strong>^1,2</strong><strong>^,</strong><strong>*, Rahul Dev Bairagi </strong><strong>²</strong><strong>, Dipa Debnath </strong><strong>²</strong><strong>, Barsha Sarker Nipa </strong><strong>³</strong><strong>, </strong><strong><br /></strong><strong>Raiyan Rahman Reon </strong><strong>²</strong><strong>, Rony Ahmed </strong><strong>⁴</strong><strong>, Tawhidur Rahman </strong><strong>⁵</strong><strong>, Shankar Sharma </strong><strong>⁶</strong><strong> and Amit Kumar Acharzo </strong><strong>²</strong></p> </div> <div class="abstract_top"> <p>¹ Department of Pharmacy, Dhaka International University, Dhaka 1212, Bangladesh</p> <p>² Pharmacy Discipline, School of Life Sciences, Khulna University, Khulna 9208, Bangladesh</p> <p>³ Department of Nutrition and Food Engineering, Faculty of Allied Health Sciences, Daffodil International University, Dhaka 1207, Bangladesh</p> <p>⁴ Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh</p> <p>⁵ Department of Pharmacy, Northern University of Bangladesh, Dhaka 1230, Bangladesh</p> <p>⁶ Department of Pharmacy, Faculty of Basic Medical and Pharmaceutical Sciences, University of Science &amp; Technology Chittagong (USTC), Chattogram 4202, Bangladesh</p> <p><strong>*</strong> Correspondence: sazzadnishan96@gmail.com; Tel.: +880-1521305611</p> </div> <div class="abstract_top"> <p>Received: 19 September 2024; Revised: 14 October 2024; Accepted: 25 October 2024; Published: 27 November 2024</p> </div> <p><strong class="label">Abstract: </strong><em>Physalis angulata </em>L. family Solanaceae, commonly known as ground cherry, cape gooseberry, or bladder cherry, has a long history of traditional use in various regions around the world. The primary goal of this study is to investigate the different pharmacological effects produced by the ethanolic<em> </em>leaf extracts of<em> Physalis angulata. </em>The leaf extract was prepared in two different dosages: 250 mg/kg body weight and 500 mg/kg body weight, which were administered according to the body weight of the mice.<em> </em>In yeast-induced pyrexia in mice, after 4 h, positive control (Paracetamol 150 mg/kg), <em>Physalis angulata</em> 250 mg/kg, <em>Physalis angulata</em> 500 mg/kg expressed temperature were 98.78 ± 0.051 °F, 97.4 ± 0.213 °F and 96.56 ± 0.177 °F respectively.<em> </em>In the evaluation of acetic acid-induced peripheral analgesic activity,<em> P. angulata </em>extract exhibited 43% and 63% inhibition of writhing at 250 mg/kg and 500 mg/kg body weight, respectively. Whereas the standard Diclofenac-Na inhibited 76% at a dose of 25 mg/kg body weight. In castor oil-induced diarrhea, plant extract inhibited defecation by 59.65% at 250 mg/kg body weight and 72.45% at 500 mg/kg b.w., whereas standard loperamide at a dose of 3 mg/kg b.w. inhibited 83.50% of defecation. Ethanolic extract of <em>Physalis angulata</em> at the dose of 300 mg/kg, 2000 mg/kg and 5000 mg/kg showed average weight 21.2 ± 1.56 gm, 21.8 ± 0.82 gm and 24.45 ± 1.51 gm respectively at 2nd day. The disc diffusion method has been adopted for the evaluation of antimicrobial activity. The ethanolic extracts of <em>Physalis angulata</em> leaf exhibited inhibitory activity against fourteen strains, including Bacillus megaterium, Salmonella paratyphi, Candida aibicans, Vibrio mimicus, and Staphylococcus aureus.</p>

2024-11-27T00:00:00+08:00 Copyright (c) 2024 by the authors.